Reacción a "Reaction to study claiming that seasonal H1N1 flu is a direct descendant of 1918 flu"

The characterisation and study of the influenza virus of the 1918 pandemic is a very interesting field. It allows us, with the current methodology, to learn more about the virus that caused a pandemic that has similarities with the one we are suffering today.

This work vindicates the importance of well-preserved archived collections, which are a source of material that allows us to update, with the development of new methodologies, information on past pathogens that can provide information applicable to the present and future.

The main limitation of the work is the number of samples used, as is normal given the difficulty of obtaining them. Despite this limitation, the methodology used to obtain the new virus sequences by mass sequencing and the evolutionary models used are adequate and allow the results presented to be obtained reliably.

The comparison of European samples prior to and during the peak of the pandemic with American samples provides information on the viral variability that could exist between pandemic viruses and the host adaptations that could have arisen.

This information allows today's virologists to monitor changes in these positions in animal viruses or during the emergence of new pandemic viruses. This work provides a description of changes that affect the activity of the virus polymerase, although we do not know if this has a real impact on virus replication, and changes that may be associated with host adaptation in the nucleoprotein and that would also improve the ability of the virus to respond to the innate immune system.

In 2014, Rambaut et al. published a paper linking the origin of the virus to the recombination of a haemagglutinin from a circulating human H1 virus with 7 segments of an avian influenza virus. This paper, which cannot rule out that hypothesis, presents a new alternative. Phylogenetic analysis, using newly available prepandemic sequences, reveals robust association of all internal genes of seasonal and pandemic viruses, except for haemagglutinin and neuraminidase. In the case of viral antigens, these segments cluster better with swine viruses than with hitherto circulating influenza viruses. The evolutionary divergence between seasonal and pandemic haemagglutinins and neuraminidases calculated with different evolutionary models shows the possibility of a different origin than proposed so far.